cDNA Cloning and Analysis of the Chicken Homolog (chApex1) of APEX Nuclease, a Multifunctional DNA Repair Enzyme

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Title
cDNA Cloning and Analysis of the Chicken Homolog (chApex1) of APEX Nuclease, a Multifunctional DNA Repair Enzyme
Author
著者 関 周司
著者(別表記) Seki Shuji
著者 Nakamura Takashi
著者(別表記) Nakamura Takashi
著者 Sarker Altaf H.
著者(別表記) Sarker Altaf H.
著者 関 雄一
著者(別表記) Seki Yuichi
著者 Ikeda Shogo
著者(別表記) Ikeda Shogo
Abstract

We have cloned and analyzed the cDNA (chApex1) for the chicken homolog of APEX nuclease, a multifunctional DNA repair enzyme. Chicken Apex cDNA fragments were first amplified from Chick Embryo Lambda cDNA Library by nested PCR (polymerase chain reaction) using primers constructed
on the basis of information of highly conserved regions of the amino acid sequences among mouse, rat and human APEX nuclease. Almost full-size chicken Apex cDNA was cloned from the Chick Embryo Lambda cDNA Library using the amplified chicken Apex cDNA fragments as a probe.
The cloned cDNA was 1751 nucleotide long encoding a protein consisting of 300 amino acids with a predicted molecular mass of 33 kDa. The amino acid sequence exhibits a high homology with the sequence of the mammalian APEX nuclease (64%, 62% and 63% identities/300 amino acids with those of the human, mouse and rat APEX nuclease, respectively). The homologies of the amino acid sequences between chicken and mammalian APEX nucleases occurred along nearly the entire length of the sequences.

Subject
460 Biology
Keyword
chApex1 cDNA cloning
chicken APEX nuclease
multifunctional DNA repair enzyme
exonuclease III homolog
chicken
Publish Date
2008
Publication Title
Chugokugakuen journal
Volume
7
Start Page
1
End Page
5
ISSN
1348-1452
NCID
AA11806612
Publisher
中国学園大学/中国短期大学
Copyright Holders
中国学園大学/中国学園短期大学
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Contents Type
Departmental Bulletin Paper
language
English
File Version
publisher