cDNA Cloning and Analysis of the Chicken Homolog of E.coli Endonuclease Ⅲ

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Title
cDNA Cloning and Analysis of the Chicken Homolog of E.coli Endonuclease Ⅲ
Author
著者 Seki Shuji
著者(別表記)
著者 H. Sarker Altaf
著者(別表記)
著者 Nakamura Takashi
著者(別表記)
著者 Seki Yuichi
著者(別表記)
著者 Ikeda Shogo
著者(別表記)
Abstract

We have cloned and analyzed the cDNA for the chicken homolog of E. coli endonuclease Ⅲ (chNthll; chicken homolog of E. coli endonuclease Ⅲ-like 1). The cDNA was isolated from a chicken embryonic cDNA library using cDNA of mouse endonuclease Ⅲ (mNthll) as a probe. The obtained cDNA was 1211 nucleotide long encoding a protein consisting of 281 amino acids with a predicted molecular mass of 32 kDa. The amino acid sequence exhibits 66% and 67%homologies with those of the human and mouse homologs, respectively, and the homologies are mostly concentrated within the C-terminal, 2/3 region of the sequence. The two motifs consisting of a helix-hairpin-helix and an iron-sulphur [4Fe-4S] cluster were also preserved in the chicken homolog suggesting similar enzymatic function in chicken cells as that of endonuclease Ⅲ in E. coli.

Subject
460 Biology
490 Medical sciences. Pharmaceutics
Keyword
chicken NthIl
cDNA cloning
endonuclease Ⅲ homolog
chicken
Publish Date
2006
Publication Title
Chugokugakuen journal
Volume
5
Start Page
23
End Page
27
ISSN
1348-1452
NCID
AA11806612
Publisher
中国学園大学/中国短期大学
Copyright Holders
中国学園大学/中国短期大学
Official URL
Contents Type
Departmental Bulletin Paper
language
English
File Version
publisher