Quantitative Determination of Ascorbic Acid in Vegetables by High-Performance Liquid Chromatography

URI http://cur-ren.cjc.ac.jp/metadata/120
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Title
Quantitative Determination of Ascorbic Acid in Vegetables by High-Performance Liquid Chromatography
Author
著者 嶋田 義弘
著者(別表記) Shimada Yosihiro
著者 高 早苗
著者(別表記) Ko Sanae
Abstract

A simple high-performance liquid chromatographic procedure was to determine levels of ascorbic acid and dehydroascorbic acid in vegetables that composed of reduction of dehydroascorbic acid to ascorbic acid by DL-homocysteine and the use of tetra-n-butylammonium bromide as an ion-pairing regent in the mobile phase. This method was used to determine the ascorbic acid, dehydroascorbic acid and total ascorbic acid content in 15 fresh vegetables. Sweet peppers and broccoli contained more than 80mg/l00g of TAA, while Chinese chives, tomatoes, Japanese radishes and carrots contained less than 20mg/l00g of TAA of edible portion. Many vegetables contained an inside level from 33 to 58mg/l00g. There is a difference in the content ratio of AA and DRAA according to vegetables.

Subject
490 Medical sciences. Pharmaceutics
Keyword
ascorbic acid
dehydroascorbic acid
vegetables
high-performance liquid chromatography
Publish Date
2006
Publication Title
Chugokugakuen journal
Volume
5
Start Page
13
End Page
16
ISSN
1348-1452
NCID
AA11806612
Publisher
中国学園大学/中国短期大学
Copyright Holders
中国学園大学/中国短期大学
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Contents Type
Departmental Bulletin Paper
language
English
File Version
publisher