Mouse Processed Pseudogenes (Apexp1 and Cbx3p1) for Apex Nuclease and Chromobox Homolog 3

Abstract

Mouse processed pseudogenes for Apex nuclease and chromobox homolog 3 were isolated from a mouse genomic library. Inserts of the three isolated clones (clones 2, 6 and 16) were sequenced and analyzed. Clones 2 and 6 were overlapping clones, and the inserts covered a 14.9 kilobase (kb) genomic fragment. The fragment contains a processed Apex pseudogene (designated as mouse Apexpl) and a processed Cbx3 pseudogene (designated as mouse Cbx3pl). These pseudogenes are located in inactive LINE (long interspersed repetitive element) sequences. Calculation of nucleotide substitution rates suggests that the non-functional mouse Apexpl arose 24 million years ago, and that the non-functional mouse Cbx3pl arose even earlier, 56 million years ago. The insert sequence of the clone 16 contains a processed Apex pseudogene (designated as mouse Apexp2) differing from the Apexpl.